關於 sfgfp ，我們在網路上蒐集到這些相關的討論、資訊與評價

#11. Part:BBa K2541401 - iGEM Parts

sfGFP (BbsI free). It is a superfolder GFP, a robustly folded version of GFP, which is BbsI restriction site free and can be used in Golden ...

#12. 030952 - CAG-Sun1/sfGFP Strain Details

030952 B6.129-Gt(ROSA)26Sor tm5.1(CAG-Sun1/sfGFP)Nat /MmbeJ These Sun1-tagged mice, backcrossed to C57BL/6J, are used in the INTACT ...

#13. Construction of pRSET-sfGFP Plasmid for Fusion-Protein ...

This study aimed to design a system for protein expression and detection using a new super-folder derivative of GFP (sfGFP) as a fusion partner, ...

#14. 7E53: Crystal structure of sfGFP complexed with ... - RCSB PDB

Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution · Structural insights into two distinct nanobodies ...

#15. Maturation time for sfGFP (super folded GFP) - BNID 110546

Maturation time for sfGFP (super folded GFP) ; Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol.

#16. sfGFP Vectors - Regular Plasmid, Lentivirus & AAV

sfGFP vectors (from $159) available in regular plasmid, lentivirus, AAV, adenovirus, & piggyBac formats from VectorBuilder.

#17. Split-wrmScarlet and split-sfGFP: tools for faster, easier ...

Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans. Jérôme Goudeau,.

#18. Live imaging and conditional disruption of native PCP activity ...

Through live imaging of endogenous sfGFP-Vangl2 expression, we report on the authentic regulation of vertebrate PCP during embryogenesis.

#19. TgBAC(cxcr4b:cxcr4b-TagRFP-sfGFP,crybb1:ECFP) - ZFIN

sfGFP is a super folder variant of GFP. Plasmid Map. None. Genomic Features. That Utilize TgBAC(cxcr4b:cxcr4b-TagRFP-sfGFP,crybb1 ...

#20. 改善三方sfGFP系统荧光信号的策略。 - X-MOL

As an elegant extension of BiFC, a tripartite superfold green fluorescent protein (sfGFP) system that has the advantages of low background ...

#21. Showing 1 - SynBioHub

sfGFP. Public. BBa_K1321337 BBa_K1321337 Version 1 (Component) ... superfolder GFP (sfGFP) ... sfGFP, green fluorescence protein reporter system.

#22. Anti-sfGFP [NbsfGFP01] - Absolute Antibody

Recombinant anti-Aequorea victoria (Water jellyfish sfGFP (Superfolder green fluorescent protein) antibody. Clone NbsfGFP01. Engineered into new species and ...

#23. Sensitivity of Superfolder GFP to Ionic Agents | PLOS ONE

Superfolder variant of the green fluorescent protein (sfGFP) became a favorite probe for examination of the unfolding–refolding processes of ...

#24. Chemical Biology Tools for Peptide and Protein Research

The sfGFP-10ams gene, amber mutations at 10 phenylalanine positions ... The sfGFP-27am gene was generated using the sfGFP gene amplified by PCR from ...

#25. sfGFP Antibody (NBP3-09058) - Novus Biologicals

View Rabbit Polyclonal anti-sfGFP Antibody (NBP3-09058). Validated Applications: WB. Validated Species: Non-species specific.

#26. Bacterial Secretion Systems, Volume II - 第 76 頁 - Google 圖書結果

levels of the full-length HrcQ-sfGFP protein (Figure 7C). Notably, wild-type protein levels of HrcQM211A -sfGFP were restored in the presence of HrcQC -mKOκ ...

#27. A guide to choosing fluorescent proteins - Abcam

Superfolder GFP (sfGFP) for example, can fold in under 10 minutes, while mOrange2 can take over four hours. Bleaching is a measure of photostability, ...

#28. In vivo selection of sfGFP variants with improved and reliable ...

Keywords: GFP, sfGFP, Thermostability, Biotechnology, Parageobacillus sp., Geobacillus sp., Thermophilic bacteria,. FACS, Protein engineering, In vivo ...

#29. sfGFP荧光蛋白的激发波长和发射波长 - 知乎专栏

sfGFP 的激发波长主峰在488nm，发射波长主峰在507nm。 上海路阳仪器有限公司现货销售进口便携式荧光蛋白激发光源，又叫荧光蛋白观察灯、GFP激发光源， ...

#30. sfGFP Rabbit Polyclonal Antibody (ER1802-92) - HUABIO

TARGET. PROTEIN NAME. sfGFP. FUNCTION. Tagging proteins with a functional sequence is ...

#31. Intermediate Filament Associated Proteins - 第 74 頁 - Google 圖書結果

We purify KASH2 as a fusion to the C terminus of 6Â His-sfGFP; this 6Â His-sfGFP–KASH2 polypeptide (sfGFP–KASH2) is first purified by Ni2+-affinity as ...

#32. Bibliographies: 'SfGFP, superfolder green fluorescent protein'

Relevant books, articles, theses on the topic 'SfGFP, superfolder green fluorescent protein.' Scholarly sources with full text pdf download.

#33. CN101830972A - 基于绿色荧光蛋白sfGFP的荧光互补系统

本发明基于superfolder GFP(sfGFP)荧光蛋白，从其第214和第215氨基酸残基分离得到两个荧光蛋白片段(sfGFPN and sfGFPC)；其中sfGFPC片段通过点突变的方法改进为假阳性 ...

#34. Crystal structure of a domain‐swapped photoactivatable ...

Crystal structure of a domain-swapped photoactivatable sfGFP variant provides evidence for GFP folding pathway. Stephanie Kesgin-Schaefer,.

#35. RDB18608 - pCW DNAseIIα-sfGFP-T2A-mCherry

pCW DNAseIIα-sfGFP-T2A-mCherry. Lentiviral expression vector of lysosomal-METRIQ (MEasurement of protein Transporting integrity by RatIo Quantification) probe.

#36. To cite pCRISPaint-T2A-sfGFP-3xP3-RFP

When publishing experiments using pCRISPaint-T2A-sfGFP-3xP3-RFP, please include the following details in your manuscript. Materials and Methods.

#37. Using Tripartite Split-sfGFP for the Study of Membrane Protein ...

Recently, the newly developed tripartite split-sfGFP method was demonstrated to detect membrane PPIs in plant cells without spurious background signals even ...

#38. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS载体_质粒图谱

优宝生物提供pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS载体,产品优质,服务快速,同时提供pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS质粒图谱,载体序列,载体抗性及操作手册等 ...

#39. Construction of pRSET-sfGFP Plasmid for Fusion-Protein ...

Keywords: sfGFP, Gene cloning, protein expression, polyclonal antibody. * Corresponding author. e-mail: : [email protected]. 1. Introduction. The green ...

#40. The Story of Free Use GFP (fuGFP) - Small Things Considered

Luckily the Coleman lab research assistant Mark Somerville was not afraid of a little legal legwork and he dived deep into the sfGFP patent. Our ...

#41. Figures and data in zGrad is a nanobody-based degron ... - eLife

(C) Schematic of zGrad-mediated target protein degradation. (D) Representative images of embryos injected with sfGFP-mAID mRNA (210 pg) only ( ...

#42. Split-wrmScarlet and split-sfGFP: tools for faster ... - bioRxiv

Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans.

#43. sfGFP-Golgi-7 vector - Ximbio

sfGFP -Golgi-7 vector. Invented at Florida State University. Datasheet. References (0). Inventor Info. Print. Info. Catalogue Number, 157529.

#44. pHrdSV40- scFv- GCN4- sfGFP- VP64 - lifescience-market.com

pHrdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS. Catalog No. PVT10950 Packing 2ug. Function Mammal Editing plasmids. Resistance Amp Screen / Strain /

#45. Supplemental Figure 1. Bipartite sfGFP designs exhibits heavy ...

Bipartite sfGFP designs exhibits heavy background signals. (A) The original design of dCas9 fused with EGFP (left).

#46. In vivo selection of sfGFP variants with improved and reliable ...

The best mutant sfGFP(N39D/A179A) showed an 885-fold enhanced mean fluorescence intensity (MFI) at 60 °C and is the most reliable reporter ...

#47. sfGFP: Superfolder green fluorescent protein - Shapeways

sfGFP : Superfolder green fluorescent protein (L2X6W4QE8) by JDDS on Shapeways. Learn more before you buy, or discover other cool products in the marketplace ...

#48. Using RiboTag and Sun1-sfGFP technologies to map Müller ...

Müller glia-specific (GLAST Cre) and neural retina (Chx10 Cre) with either RPL22:HA f/+ or Sun1-sfGFP f/+ mice were injected with TNFα to induce LIF expression.

#49. In vivo selection of sfGFP variants with improved ... - PubAg - USDA

An undirected mutagenesis approach coupled to fluorescence-activated cell sorting allowed the isolation of sfGFP variants that were extremely well expressed in ...

#50. Plant Biotechnol. 32(1): 81-87 (2015) - J-Stage

variants, GFP-S65T, eGFP, frGFP and sfGFP, with reference to the fluorescence intensity in Arabidopsis protoplasts, and.

#51. Strategies to improve the fluorescent signal of the tripartite ...

Two bipartite sfGFP systems, splitting at 157/158 or 173/174, were used in the comparison. Green fluorescent protein (GFP) is the first successful trial among ...

#52. GAF-CaMP3–sfGFP, An Enhanced Version of the Near ... - MDPI

GAF-CaMP3–sfGFP, An Enhanced Version of the Near-Infrared Genetically Encoded Positive Phytochrome-Based Calcium Indicator for the Visualization of Neuronal ...

#53. 6dq1 - sfGFP N149 mutated to 4-nitro-L-phenylalanine

Descriptor, sfGFP, SODIUM ION (3 entities in total). Functional Keywords, super folder gfp, 4-nitro-l-phenylalanine, unnatural amino acid, ...

#54. Effects of pH and organic solvents on superfolder green ...

Emission spectra of sfGFP were measured at varying dilutions in several organic solutions including 99.9% methanol (MeOH), 99.9% acetonitrile ( ...

#55. pAJGb2-sfgfp II (PhlF Design) - Benchling

Full sequence for pAJGb2-sfgfp II (PhlF Design) shared on Benchling.

#56. sfGFP-pBAD | Addgene 54519 product information - Labome

Catalog Number : 54519 ; Product Name : sfGFP-pBAD ; article : doi, 10.1038/nbt1172. id, 28196437. pubmed_id, 16369541 ; bacterial resistance : Ampicillin ; cloning ...

#57. Banana sensor sfGFP test - protocols.io

Based on iGEM team EPFL 2019, we try to define a concentration of Toehold switch and trigger DNA sequence which has a high protein expression(sfGFP in this case) ...

#58. Using Tripartite Split-sfGFP for the Study of ... - Springer Link

Here we describe a protocol for using the ß-Estradiol-inducible tripartite split-sfGFP assay for side-by-side analyses of in vivo PPI along with ...

#59. Full article: EXPRESSION IN E. coli AND PURIFICATION OF ...

Thus, the use of sfGFP has made it possible to avoid formation of inclusion bodies (IB) during the synthesis of recombinant fusion proteins and ...

#60. Expressıon Strategy of Soluble Recombınant Human TGF-β3 ...

Superfolder GFP (sfGFP) is one of the fusion tags used to increase the solubility of the fusion partner in E. coli. In this study, TGF-β3 with sfGFP fusion ...

#61. Supplementary information for - The Royal Society of Chemistry

Figure S2: Expression of sfGFP reporters with increasing N-terminal UAG codons using a pEVOL plasmid encoding an M. jannaschii-derived polyspecific tyrosyl tRNA ...

#62. pHR-scFv-GCN4-sfGFP-GB1-dWPRE vector map and sequence

Provide pHR-scFv-GCN4-sfGFP-GB1-dWPRE vector/plasmid map, full length sequence, antibiotic resistance, size and other information.

#63. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (OVT2679)

Use pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS for functional genomics research and drug discovery study.

#64. PERspectives | Parts

BBa_K4294754, Composite, 36ntLuxI - sfGFP Transcriptional unit with the Ath4 Synthetic RBS (TetR Regulated), Aristotelis Anastopoulos, 969.

#65. Creation, Expression, Purification and Characterization of GFP

In molecular biology, this law is employed frequently for determining the concentration of DNA or protein in a sample. In the case of sfGFP, we ...

#66. In vivo selection of sfGFP variants with improved and ... - Gale

Keywords: GFP, sfGFP, Thermostability, Biotechnology, Parageobacillus sp., Geobacillus sp., Thermophilic bacteria, FACS, Protein engineering, ...

#67. Chromium responsive promoter with UTR+RBS and sfGFP ...

This BioBrick encodes for "Chromium responsive promoter with UTR+RBS and sfGFP" and its validation status is reported as: "Works".

#68. Split aminoacyl-tRNA synthetases for proximity-induced stop ...

We measured sfGFP fluorescence in cells grown in the presence and absence of 2 mM mIF. Under these conditions, sfGFP production should occur only if the ...

#69. 的sfGFP-N1 （质粒＃54737） - betway必威官网平台

载体骨架. 的sfGFP-N1. （搜索矢量数据库） · 骨干大小（bp）的4750 · 矢量型. 哺乳动物表达 · 可选择标记. 新霉素（G418与选择） · 标签/融合蛋白. 的sfGFP（C终端上骨架） ...

#70. La microscopie pour comprendre la propagation de résistance ...

Vidéo : Imagerie microfluidique en temps réel montrant la production à partir du plasmide d'une protéine verte (SsbF-sfGFP) une fois le ...

#71. super folder green fluorescent protein (sfGFP) - Frontiers

This page contains Frontiers open-access articles about super folder green fluorescent protein (sfGFP)

#72. Comment les bactéries propagent-elles leur résistance aux ...

verte (SsbF-sfGFP) une fois le plasmide transféré dans la bactérie ... fluorescence verte SsbF-sfGFP et rouge ParB-mCh; SsbF-sfGFP ParB-mCh: ...

#73. Mineralization of titania with sfGFP-Car9 variants

In this work, we investigate how variants of superfolder green fluorescent protein (sfGFP) incorporating a panel of solid binding peptides (SBPs) modulate ...

#74. Full Dex - izebs

The full-length dex-1p::sfgfp::dex-1 construct rescues the lateral alae . National Pokédex National Pokédex | 001-151 | 152-251 | 252-386 | 387-493 ...

#75. Ff14sb - Jcdeutschland

... the vibrational reporter unnatural amino acid (UAA) 4-cyano-l-phenylalanine (pCNPhe) in the model system superfolder green fluorescent protein (sfGFP).

#76. Les riborégulateurs synthétiques détectent les parties du ...

Les commutateurs ont été clones en aval du promoteur T7-LacO et en amont du gène rapporteur sfGFP. Les deux constructions ont été ...

#77. rocket popz strain

Expression of sfGFP-PodJ from the. Farmer and the Felon: Farmer Felon | Rocket Popz Flower - Eighth | Leafly Get details and read the latest customer ...

#78. sfgfp gene | A comparison of the fluorescence dynamics of single ...

What is the mutation rate of the sfGFP gene? In an undirected, error-prone PCR approach, we randomly mutagenized the sfGFP (Sp) gene, thereby achieving a DNA ...

#79. Full Dex - Gloser Mietpark

The full-length dex-1p::sfgfp::dex-1 construct rescues the lateral alae . Pokemon Sword and Shield All Pokemon / 100% Full Pokedex ShowcaseFor this video I ...